Laboratory Research Associate
The University of California, San Diego
About this position
Position Description
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 60 post- doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined, and are integral to the goals of the department. The Department has undergone significant growth in recent years with a consolidated budget of approximately $106 million including sponsored projects expenditures of approximately $54 million and clinical revenue of over $ 52 million. This expansion of the Department’s research and clinical portfolio is expected to continue in the next few years. The Department’s 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine. In 2001, physicians and leadership of the University of California, San Diego (UCSD), Rady Children's, and Children's Specialists of San Diego (CSSD) unified pediatric patient care, research, education and community service programs, creating a university-affiliated children's health system to serve the region. Over the past two decades, an extensive list of joint programs has developed, with many physicians and researchers playing a role at both institutions. This affiliation has consolidated the clinical, teaching, research and public service programs of the UCSD School of Medicine's Department of Pediatrics with San Diego's only health care system dedicated to the health and well-being of children. In 2009, this partnership was furthered by the creation of Rady Children’s Specialists of San Diego, a Medical Foundation, and is now recognized as having global leaders in research, technology, translational medicine, education and clinical excellence. Under general supervision, the incumbent will independently perform and lead experiments related to hematopoietic stem cell and erythroid progenitor cell biology. The incumbent will isolate and perform ex vivo culture of both mouse and human hematopoietic stem and progenitor cells, and will also perform in vivo transplantation of isolated mouse and human hematopoietic stem and progenitor cells. The incumbent will produce retroviral and lentiviral vector and conduct CRISPR gene knockout followed by assessment with flow cytometry of both intracellular and cell surface proteins and RNA-sequencing. Performs other duties as assigned.
Qualifications
Theoretical knowledge of Molecular/Cellular Biology or a related field, as typically attained with an advanced degree, or an equivalent combination of education and experience. Experience with generation of retroviral vectors and lentiviral vectors. Experience with molecular cloning. Experience with CRISPR activation and CRISPR gene knockout. Experience with purification of red blood cell extracellular vesicles. Experience with advanced maintenance of cytoflex flow cytometer with plate loader. Experience with independent operation of BD Facs Symphony cell sorter. Experience with methanol cell permeation and intracellular flow cytometry and phospho flow cytometry. Experience isolating mouse and human burst forming unit erythroid cells and colony forming unit erythroid cells. Experience with tail vein and retroorbital injection of hematopoietic stem cells and hematopoietic progenitor cells. Experience with creation and maintenance of stable 3T3 cell lines overexpressing c-Kit and CXCL12. Experience with creation and maintenance of stable 3T3 cell lines overexpressing CRISPR-A. Experience with creation and maintenance of stable 3T3 cell lines with CRISPR activation of Sca1. Experience with flow cytometric analysis of primary erythroid differentiation culture. Demonstrated ability to follow verbal and/or written instructions, including follow-up on previously begun procedures. Demonstrated accurate record data and enter into appropriate medium. Demonstrated experience mentoring and overseeing undergraduates and postdoctoral fellows. Experience with computer software such as Excel and Prism to analyze data, store results and compute raw data. Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Demonstrated ability to read, comprehend and discuss research materials.