Life Science Research Professional 2
Stanford University
About this position
Position Description
Stanford University is seeking a Life Science Research Professional 2 to perform translational research in the Ramakrishna Laboratory to learn from and optimize active and ongoing cellular therapy clinical trials at Stanford and beyond. The Ramakrishna laboratory focuses on optimizing chimeric antigen receptor T cell (CAR-T) therapies for children with cancer. The lab performs multi-parametric analyses on patient samples from cellular immunotherapy clinical trials and validates novel manipulatable biomarkers to optimize CAR T cell activity in patients. We utilize multi-dimensional analyses of flow cytometry/CyTOF/CyTEK single cell proteomics, 10 x genomics single cell transcriptomics, and ATAC-seq epigenomics to identify contributors to CAR-T success or failure in patients. These correlative studies are critical for the development and assessment of new cell-based immunotherapies. The successful candidate will design, plan, and execute experiments to identify predictors and/or drivers of CAR-T activity in patients. Experiments may include, but are not limited to 1) Developing and validating sophisticated, multi-dimensional flow-based panels; 2) Cloning of novel CAR constructs for testing in the lab; 3) Performing in vitro experiments, such as co-culture, incucyte, and Elisa assays to validate biomarkers identified in multi-dimensional analyses 4) Analyzing flow, cytokine, qPCR, and other data streams from clinical trials in the context of clinical response 5) Assess CAR-T optimization approaches in mouse models. Other job duties include maintaining excellent lab records, report weekly to supervisor, effectively communicate experimental results to the team and the principal investigator, and train other lab personnel as needed. This work is highly collaborative within the larger institution of the Stanford Cancer Cellular Therapy Team, and so the candidate is expected to engage in team science, working with post-docs, other LSRPs, students, and collaborating scientists. Duties include: · Independently conduct a portion of the research project(s). Make recommendations on experimental design and/or research direction to principal investigator. · Independently conduct complex (lab and/or field) experiments to gather data for a portion of a major research project. · Interpret and analyze results, often where few guidelines exist, and make modifications to existing procedures and protocols. · Develop new research protocols where appropriate protocols are not described in the literature or where modification or adaptation of standard procedures and protocols is required. · Participate in multidisciplinary teams across different faculties or schools. · Perform ongoing literature review to remain current with new procedures and related research; apply to ongoing research and development of new protocols. · Contribute substantively to the preparation of papers for publication, especially in the results section, and to publication of findings. Present ongoing work to colleagues and/or academic conferences. · Oversee supply and equipment budget under supervision of principal investigator or business manager. · Work with health and safety department to ensure lab complies with regulations and all required trainings. · Supervise (either formally or informally) staff or students as needed, including oversight and direction on techniques, as well as consultation on project work. Serve as a technical resource for other research staff. · Oversee the daily activities of the laboratory including lab maintenance; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. May assist with purchasing laboratory equipment. *- Other duties may also be assigned Stanford University provides pay ranges representing its good faith estimate of what the University reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs. The pay range for this position working in the California Bay area is $85,376.00 to $99,581.00 annually. DESIRED QUALIFICATIONS: Experience performing flow cytometry, cell sort, and knowledge using Diva, Flowjo or Cytobank, Graph-pad Prism Bachelor’s degree in biological sciences, immunology, or chemistry with 2 years’ experience Master’s degree in biological sciences, immunology, or chemistry EDUCATION & EXPERIENCE (REQUIRED): Bachelor's degree in related scientific field and two years of relevant work experience; or Master’s degree in a related scientific field. KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED): · Comprehensive understanding of scientific principles. · General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications. · Strong analytical skills and excellent judgment. · Ability to maintain detailed records of experiments and outcomes. · Ability to work under deadlines with general guidance is essential. · Excellent organizational skills and demonstrated ability to accurately complete detailed work. CERTIFICATIONS & LICENSES: Add here PHYSICAL REQUIREMENTS*: · Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds. · Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts. · Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls. · Must have correctible vision to perform duties of the job. · Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours. · Ability to lift heavy objects weighing up to 50 pounds. · Ability to work in a dusty, dirty, and odorous environment. · Position may require repetitive motion. · Add or subtract physical requirements based on the requirements of your specific job. (remove this statement for posting) *- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job. WORKING CONDITIONS: · May require working in close proximity to blood borne pathogens. · May require work in an environment where animals are used for teaching and research. · Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present. · Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator. · May require extended or unusual work hours based on research requirements and business needs. Additional Information Schedule: Full-time Job Code: 4952 Employee Status: Regular Grade: G Department URL: http://pediatrics.stanford.edu/ Requisition ID: 108899 Work Arrangement : On Site
Qualifications
DESIRED QUALIFICATIONS: Experience performing flow cytometry, cell sort, and knowledge using Diva, Flowjo or Cytobank, Graph-pad Prism Bachelor’s degree in biological sciences, immunology, or chemistry with 2 years’ experience Master’s degree in biological sciences, immunology, or chemistry EDUCATION & EXPERIENCE (REQUIRED): Bachelor's degree in related scientific field and two years of relevant work experience; or Master’s degree in a related scientific field. KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED): · Comprehensive understanding of scientific principles. · General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications. · Strong analytical skills and excellent judgment. · Ability to maintain detailed records of experiments and outcomes. · Ability to work under deadlines with general guidance is essential. · Excellent organizational skills and demonstrated ability to accurately complete detailed work. CERTIFICATIONS & LICENSES: Add here PHYSICAL REQUIREMENTS*: · Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds. · Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts. · Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls. · Must have correctible vision to perform duties of the job. · Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours. · Ability to lift heavy objects weighing up to 50 pounds. · Ability to work in a dusty, dirty, and odorous environment. · Position may require repetitive motion. · Add or subtract physical requirements based on the requirements of your specific job. (remove this statement for posting) *- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job. WORKING CONDITIONS: · May require working in close proximity to blood borne pathogens. · May require work in an environment where animals are used for teaching and research. · Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present. · Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator. · May require extended or unusual work hours based on research requirements and business needs.
Job Location
🔍 School of Medicine, Stanford, California, United States